Article
Typing of bacteriophages by randomly amplified polymorphic DNA (RAPD)-PCR to assess genetic diversity.
FEMS microbiology letters - 1 Sept 2011
Gutiérrez Diana, Martín-Platero Antonio M, Rodríguez Ana, Martínez-Bueno Manuel, García Pilar, Martínez Beatriz
Abstract excerpt
The recent boom in phage therapy and phage biocontrol requires the design of suitable cocktails of genetically different bacteriophages. The current methods for typing phages need significant quantities of purified DNA, may require a priori genetic information and are cost and time consuming. We have evaluated the randomly amplified polymorphic DNA (RAPD)-PCR technique to produce unique and reproducible band...
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