Article
Spectral Tuning Mechanism of Primate Blue-sensitive Visual Pigment Elucidated by FTIR Spectroscopy.
Scientific reports - 7 Jul 2017
Katayama Kota, Nonaka Yuki, Tsutsui Kei, Imai Hiroo, Kandori Hideki
Abstract excerpt
Protein-bound water molecules are essential for the structure and function of many membrane proteins, including G-protein-coupled receptors (GPCRs). Our prior work focused on studying the primate green- (MG) and red- (MR) sensitive visual pigments using low-temperature Fourier transform infrared (FTIR) spectroscopy, which revealed protein-bound waters in both visual pigments. Although the internal waters are...
Topics
- Animals
- Cloning, Molecular
- Color Vision
- Gene Expression
- Genetic Vectors
- HEK293 Cells
- Haplorhini
- Humans
- Light
- Mutagenesis, Site-Directed
- Mutation
- Recombinant Proteins
- Retinal Pigments
- Retinaldehyde
- Schiff Bases
- Spectroscopy, Fourier Transform Infrared
- Water
