Article
DNA isolation protocol effects on nuclear DNA analysis by microarrays, droplet digital PCR, and whole genome sequencing, and on mitochondrial DNA copy number estimation.
PloS one - 1 Jan 2017
Nacheva Elizabeth, Mokretar Katya, Soenmez Aynur, Pittman Alan M, Grace Colin, Valli Roberto, Ejaz Ayesha, Vattathil Selina, Maserati Emanuela, Houlden Henry, Taanman Jan-Willem, Schapira Anthony H, Proukakis Christos
Abstract excerpt
Potential bias introduced during DNA isolation is inadequately explored, although it could have significant impact on downstream analysis. To investigate this in human brain, we isolated DNA from cerebellum and frontal cortex using spin columns under different conditions, and salting-out. We first analysed DNA using array CGH, which revealed a striking wave pattern suggesting primarily GC-rich cerebellar losses,...
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