Article
Biosynthesis of isonitrile lipopeptides by conserved nonribosomal peptide synthetase gene clusters in Actinobacteria.
Proceedings of the National Academy of Sciences of the United States of America - 3 Jul 2017
Harris Nicholas C, Sato Michio, Herman Nicolaus A, Twigg Frederick, Cai Wenlong, Liu Joyce, Zhu Xuejun, Downey Jordan, Khalaf Ryan, Martin Joelle, Koshino Hiroyuki, Zhang Wenjun
Abstract excerpt
A putative lipopeptide biosynthetic gene cluster is conserved in many species of Actinobacteria, including Mycobacterium tuberculosis and M. marinum, but the specific function of the encoding proteins has been elusive. Using both in vivo heterologous reconstitution and in vitro biochemical analyses, we have revealed that the five encoding biosynthetic enzymes are capable of synthesizing a family of isonitrile...
Topics
- Actinobacteria
- Biological Transport
- Catalysis
- Chromatography
- Chromatography, Ion Exchange
- Escherichia coli
- Fatty Acids
- Gene Deletion
- Lipopeptides
- Lysine