Article
An optimized protocol for the generation and functional analysis of human mast cells from CD34+ enriched cell populations.
Journal of immunological methods - 1 Sept 2017
Yin Yuzhi, Bai Yun, Olivera Ana, Desai Avanti, Metcalfe Dean D
Abstract excerpt
The culture of mast cells from human tissues such a cord blood, peripheral blood or bone marrow aspirates has advanced our understanding of human mast cells (huMC) degranulation, mediator production and response to pharmacologic agents. However, existing methods for huMC culture tend to be laborious and expensive. Combining technical approaches from several of these protocols, we designed a simplified and more...
Topics
- Antigens, CD34
- Biomarkers
- Budgets
- Cell Degranulation
- Cell Differentiation
- Cell Lineage
- Cell Proliferation
- Cell Separation
- Cell Shape
- Cells, Cultured
- Cost Savings
- Cost-Benefit Analysis
- Cryopreservation
- Culture Media