Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

An optimized protocol for the generation and functional analysis of human mast cells from CD34+ enriched cell populations.

Journal of immunological methods - 1 Sept 2017

Yin Yuzhi, Bai Yun, Olivera Ana, Desai Avanti, Metcalfe Dean D

Abstract excerpt

The culture of mast cells from human tissues such a cord blood, peripheral blood or bone marrow aspirates has advanced our understanding of human mast cells (huMC) degranulation, mediator production and response to pharmacologic agents. However, existing methods for huMC culture tend to be laborious and expensive. Combining technical approaches from several of these protocols, we designed a simplified and more...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Antigens, CD34
Share to Cell Degranulation

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Cryopreservation

Share to Interleukin-3

Share to Mast Cells

Share to Receptors, IgE

Share to Stem Cell Factor

Share to Workflow

Source

Open in PubMed Open full text

Identifiers

PMID: 28629733
DOI: 10.1016/j.jim.2017.06.003
PMCID: PMC5654741

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

An optimized protocol for the generation and functional analysis of human mast cells from CD34+ enriched cell populations.PMID 28629733Read ↗

Buffy coat preparation is a convenient source of progenitors for culturing mature human mast cells.Semantic proximity Read ↗

Human induced pluripotent stem cell-derived mast cells useful for in vitro mast cell activation assay exhibiting phenotypes and morphological characteristics of human mast cells.Semantic proximity Read ↗

Generation of a human allergic mast cell phenotype from CD133(+) stem cells.Semantic proximity Read ↗

Generation of a Human Allergic Mast Cell Phenotype from CD133+ Stem Cells.Semantic proximity Read ↗

Phenotypic and functional characterization of in vitro cultured human mast cells.Semantic proximity Read ↗

Culturing cells with mast cell phenotype and function: Comparison of peripheral blood and bone marrow as a source.Semantic proximity Read ↗

Generation of a considerable number of functional mast cells with a high basal level of FcepsilonRI expression from cord blood CD34+ cells by co-culturing them with bone marrow stromal cell line under serum-free conditions.Semantic proximity Read ↗

KIT D816V Mast Cells Derived from Induced Pluripotent Stem Cells Recapitulate Systemic Mastocytosis Transcriptional Profile.Semantic proximity Read ↗

A ten-year retrospective analysis of the distribution, use and phenotypic characteristics of the LAD2 human mast cell line.Semantic proximity Read ↗

Early development and functional properties of tryptase/chymase double-positive mast cells from human pluripotent stem cells.Semantic proximity Read ↗

Origin, maturation and recruitment of mast cell precursorsSemantic proximity Read ↗

Lin− CD34hi CD117int/hi FcεRI+ cells in human blood constitute a rare population of mast cell progenitorsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.