Article
Phenotypic and functional characterization of in vitro cultured human mast cells.
Cytometry. Part B, Clinical cytometry - 1 Sept 2017
Cop N, Decuyper I I, Faber M A, Sabato V, Bridts C H, Hagendorens M M, De Winter B Y, De Clerck L S, Ebo D G
Abstract excerpt
BACKGROUND: Mast cell progenitor cells, derived from CD34+ hematopoietic stem cells, enter the circulation and subsequently mucosal or connective tissues where they mature to mast cells. Upon activation, mast cells increase the expression of activation markers, e.g. CD63, and release histamine amongst other mediators. Traditionally, release of these mediators is quantified using assays measuring their...
Topics
- Antibodies, Anti-Idiotypic
- Cell Culture Techniques
- Cell Differentiation
- Cells, Cultured
- Flow Cytometry
- Hematopoietic Stem Cells
- Histamine
- Histamine Release
- Humans
- Mast Cells
- Phenotype