Article
Laboratory Evolution of a Biotin-Requiring Saccharomyces cerevisiae Strain for Full Biotin Prototrophy and Identification of Causal Mutations.
Applied and environmental microbiology - 15 Aug 2017
Bracher Jasmine M, de Hulster Erik, Koster Charlotte C, van den Broek Marcel, Daran Jean-Marc G, van Maris Antonius J A, Pronk Jack T
Abstract excerpt
Biotin prototrophy is a rare, incompletely understood, and industrially relevant characteristic of Saccharomyces cerevisiae strains. The genome of the haploid laboratory strain CEN.PK113-7D contains a full complement of biotin biosynthesis genes, but its growth in biotin-free synthetic medium is extremely slow (specific growth rate [μ] ≈ 0.01 h-1). Four independent evolution experiments in repeated batch cultures...
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