Article
RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination.
Molecular cell - 1 Jun 2017
Inano Shojiro, Sato Koichi, Katsuki Yoko, Kobayashi Wataru, Tanaka Hiroki, Nakajima Kazuhiro, Nakada Shinichiro, Miyoshi Hiroyuki, Knies Kerstin, Takaori-Kondo Akifumi, Schindler Detlev, Ishiai Masamichi, Kurumizaka Hitoshi, Takata Minoru
Abstract excerpt
RFWD3 is a recently identified Fanconi anemia protein FANCW whose E3 ligase activity toward RPA is essential in homologous recombination (HR) repair. However, how RPA ubiquitination promotes HR remained unknown. Here, we identified RAD51, the central HR protein, as another target of RFWD3. We show that RFWD3 polyubiquitinates both RPA and RAD51 in vitro and in vivo. Phosphorylation by ATR and ATM kinases is...
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