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Article

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro.

Journal of visualized experiments : JoVE - 17 May 2017

Jia Zhaofeng, Liang Yujie, Ma Bin, Xu Xiao, Xiong Jianyi, Duan Li, Wang Daping

Abstract excerpt

The dedifferentiation of hyaline chondrocytes into fibroblastic chondrocytes often accompanies monolayer expansion of chondrocytes in vitro. The global DNA methylation level of chondrocytes is considered to be a suitable biomarker for the loss of the chondrocyte phenotype. However, results based on different experimental methods can be inconsistent. Therefore, it is important to establish a precise, simple, and...

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PMID: 28570531
DOI: 10.3791/55565
PMCID: PMC5607994

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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