Article
Mechanistic Insight from Calorimetric Measurements of the Assembly of the Binuclear Metal Active Site of Glycerophosphodiesterase (GpdQ) from Enterobacter aerogenes.
Biochemistry - 5 Jul 2017
Pedroso Marcelo M, Ely Fernanda, Carpenter Margaret C, Mitić Nataša, Gahan Lawrence R, Ollis David L, Wilcox Dean E, Schenk Gerhard
Abstract excerpt
Glycerophosphodiesterase (GpdQ) from Enterobacter aerogenes is a binuclear metallohydrolase with a high affinity for metal ions at its α site but a lower affinity at its β site in the absence of a substrate. Isothermal titration calorimetry (ITC) has been used to quantify the Co(II) and Mn(II) binding affinities and thermodynamics of the two sites in wild-type GpdQ and two mutants, both in the absence and in the...
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