Article
Coordination sphere of the third metal site is essential to the activity and metal selectivity of alkaline phosphatases.
Protein science : a publication of the Protein Society - 1 Jan 2010
Koutsioulis Dimitris, Lyskowski Andrzej, Mäki Seija, Guthrie Ellen, Feller Georges, Bouriotis Vassilis, Heikinheimo Pirkko
Abstract excerpt
Alkaline phosphatases (APs) are commercially applied enzymes that catalyze the hydrolysis of phosphate monoesters by a reaction involving three active site metal ions. We have previously identified H135 as the key residue for controlling activity of the psychrophilic TAB5 AP (TAP). In this article, we describe three X-ray crystallographic structures on TAP variants H135E and H135D in complex with a variety of...
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