Article
Parallel imaging of Drosophila embryos for quantitative analysis of genetic perturbations of the Ras pathway.
Disease models & mechanisms - 1 Jul 2017
Goyal Yogesh, Levario Thomas J, Mattingly Henry H, Holmes Susan, Shvartsman Stanislav Y, Lu Hang
Abstract excerpt
The Ras pathway patterns the poles of the Drosophila embryo by downregulating the levels and activity of a DNA-binding transcriptional repressor Capicua (Cic). We demonstrate that the spatiotemporal pattern of Cic during this signaling event can be harnessed for functional studies of mutations in the Ras pathway in human diseases. Our approach relies on a new microfluidic device that enables parallel imaging of...
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