Exploring the Amino Acid Residue Requirements of the RNA Polymerase (RNAP) α Subunit C-Terminal Domain for Productive Interaction between Spx and RNAP of Bacillus subtilis.

Abstract excerpt

Bacillus subtilis Spx is a global transcriptional regulator that is conserved among Gram-positive bacteria, in which Spx is required for preventing oxidatively induced proteotoxicity. Upon stress induction, Spx engages RNA polymerase (RNAP) through interaction with the C-terminal domain of the rpoA-encoded RNAP α subunit (αCTD). Previous mutational analysis of rpoA revealed that substitutions of Y263 in αCTD...

Topics

• Alleles
• Amino Acid Sequence
• Bacillus subtilis
• Bacterial Proteins
• DNA-Directed RNA Polymerases
• Gene Expression Regulation, Bacterial
• Genotype
• Models, Molecular
• Protein Conformation
• Protein Domains