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Development of a Japanese encephalitis virus-like particle vaccine in silkworms using codon-optimised prM and envelope genes

1 Apr 2017

Abstract excerpt

We have successfully prepared a Japanese encephalitis virus (JEV) − Nakayama virus like particle (NVLP) vaccine using synthetic codon-optimized prM and E genes. The expression of the recombinant JEV Nakayama-BmNPV (JEV-NNPV) virus was determined in infected silkworm Bm-N cells by fluorescence and Western blot analysis. The recombinant was inoculated into silkworm pupae and the yield of Nakayama VLP (NVLP) reached...

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PMID: 28435908
DOI: 10.1016/j.heliyon.2017.e00286

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