Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

The C-terminal dsRNA-binding domain of Drosophila Dicer-2 is crucial for efficient and high-fidelity production of siRNA and loading of siRNA to Argonaute2.

RNA (New York, N.Y.) - 1 Jul 2017

Kandasamy Suresh K, Zhu Li, Fukunaga Ryuya

Abstract excerpt

Drosophila Dicer-2 efficiently and precisely produces 21-nucleotide (nt) siRNAs from long double-stranded RNA (dsRNA) substrates and loads these siRNAs onto the effector protein Argonaute2 for RNA silencing. The functional roles of each domain of the multidomain Dicer-2 enzyme in the production and loading of siRNAs are not fully understood. Here we characterized Dicer-2 mutants lacking either the N-terminal...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Animals, Genetically Modified
Share to Argonaute Proteins

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Drosophila Proteins

Share to In Vitro Techniques

Share to Protein Domains

Share to Ribonuclease III

Share to RNA Helicases

Share to RNA, Small Interfering

Source

Open in PubMed Open full text

Identifiers

PMID: 28416567
DOI: 10.1261/rna.059915.116
PMCID: PMC5473147

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

The C-terminal dsRNA-binding domain of Drosophila Dicer-2 is crucial for efficient and high-fidelity production of siRNA and loading of siRNA to Argonaute2.PMID 28416567Read ↗

Functional analysis of dicer-2 missense mutations in the siRNA pathway of Drosophila.Semantic proximity Read ↗

Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways.Semantic proximity Read ↗

The RNAi pathway initiated by Dicer-2 in Drosophila.Semantic proximity Read ↗

R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway.Semantic proximity Read ↗

R2D2 organizes small regulatory RNA pathways in Drosophila.Semantic proximity Read ↗

In vitro studies provide insight into effects of Dicer-2 helicase mutations in Drosophila melanogaster.Semantic proximity Read ↗

Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes.Semantic proximity Read ↗

Dicer's helicase domain discriminates dsRNA termini to promote an altered reaction mode.Semantic proximity Read ↗

Mutational analysis of Aedes aegypti Dicer 2 provides insights into the biogenesis of antiviral exogenous small interfering RNAsSemantic proximity Read ↗

Dicer-2-Dependent Generation of Viral DNA from Defective Genomes of RNA Viruses Modulates Antiviral Immunity in InsectsSemantic proximity Read ↗

Dicer-2 Processes Diverse Viral RNA SpeciesSemantic proximity Read ↗

Secondary structure RNA elements control the cleavage activity of DICER.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.