Article
In vitro studies provide insight into effects of Dicer-2 helicase mutations in Drosophila melanogaster.
RNA (New York, N.Y.) - 1 Dec 2020
Donelick Helen M, Talide Loïc, Bellet Matthieu, Aruscavage P Joseph, Lauret Emilie, Aguiar Eric R G R, Marques Joao T, Meignin Carine, Bass Brenda L
Abstract excerpt
In vitro, Drosophila melanogaster Dicer-2 (Dcr-2) uses its helicase domain to initiate processing of dsRNA with blunt (BLT) termini, and its Platform•PAZ domain to initiate processing of dsRNA with 3' overhangs (ovrs). To understand the relationship of these in vitro observations to roles of Dcr-2 in vivo, we compared in vitro effects of two helicase mutations to their impact on production of endogenous and viral...
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