Article
Generation of human iPSCs from urine derived cells of a non-affected control subject.
Stem cell research - 1 Jan 2017
Afzal Muhammad Zeeshan, Gartz Melanie, Klyachko Ekaterina A, Khan Sadiya Sana, Shah Sanjiv J, Gupta Sweta, Shapiro Amy D, Vaughan Douglas E, Strande Jennifer L
Abstract excerpt
We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at...
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