Article
Genetic recombination in Bacillus subtilis 168: effects of recU and recS mutations on DNA repair and homologous recombination.
Journal of bacteriology - 1 Jul 1998
Fernández S, Sorokin A, Alonso J C
Abstract excerpt
Bacillus subtilis recombination-deficient mutants were constructed by inserting a selectable marker (cat gene) into the yppB and ypbC coding regions. The yppB:cat and ypbC:cat null alleles rendered cells sensitive to DNA-damaging agents, impaired plasmid transformation (25- and 100-fold), and mod...
Topics
- Bacillus subtilis
- Chloramphenicol O-Acetyltransferase
- DNA Damage
- DNA Repair
- Genes, Bacterial
- Genetic Complementation Test
- Genotype
- Methyl Methanesulfonate
- Mutagenesis
- Phenotype
- Plasmids
- Recombinant Proteins
- Recombination, Genetic
- Transformation, Bacterial