Expression and purification of a recombinant metal-binding T4 lysozyme fusion protein.

Abstract excerpt

Periplasmic expression of recombinant proteins presents many potential benefits that may aid recovery of the protein product. Muramidases are the preferred agents in effecting selective release of recombinant proteins from the periplasm of E. coli and other Gram negative bacteria. Unfortunately cost restricts the use of pure lytic enzymes at large-scale and their removal as process contaminants adds to later...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Cysteine
• Share to Enzyme Stability
• Share to Escherichia coli