Article
YfilerⓇ Plus population samples and dilution series: stutters, analytic thresholds, and drop-out probabilities.
International journal of legal medicine - 1 Nov 2017
Andersen Mikkel Meyer, Mogensen Helle Smidt, Eriksen Poul Svante, Morling Niels
Abstract excerpt
The Yfiler Ⓡ Plus Amplification Kit amplifies 27 Y chromosomal small tandem repeat (STR) markers. The kit has five-fluorescent dye chemistry and the improved PCR buffer system of modern STR kits. We validated the kit for accredited investigations of crime scene samples by a thorough study of kit dynamics and performance. We determined dye-dependent analytical thresholds by receiver operating characteristics (ROC)...
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