Article
Signature-tagged mutagenesis screening revealed a novel smooth-to-rough transition determinant of Salmonella enterica serovar Enteritidis.
BMC microbiology - 3 Mar 2017
Jiao Yang, Guo Rongxian, Tang Peipei, Kang Xilong, Yin Junlei, Wu Kaiyue, Geng Shizhong, Li Qiuchun, Sun Jun, Xu Xiulong, Zhou Xiaohui, Gan Junji, Jiao Xinan, Liu Xiufan, Pan Zhiming
Abstract excerpt
BACKGROUND: Salmonella enterica serovar Enteritidis (S. Enteritidis) has emerged as one of the most important food-borne pathogens for humans. Lipopolysaccharide (LPS), as a component of the outer membrane, is responsible for the virulence and smooth-to-rough transition in S. Enteritidis. In this study, we screened S. Enteritidis signature-tagged transposon mutant library using monoclonal antibody against somatic...
Topics
- Agglutination Tests
- Animals
- Antibodies, Monoclonal
- Bacterial Proteins
- DNA Transposable Elements
- Disease Models, Animal
- Enzyme-Linked Immunosorbent Assay
- Genes, Bacterial
- Lethal Dose 50