Article
Inhibition of CRISPR/Cas9-Mediated Genome Engineering by a Type I Interferon-Induced Reduction in Guide RNA Expression.
Biological & pharmaceutical bulletin - 1 Jan 2017
Machitani Mitsuhiro, Sakurai Fuminori, Wakabayashi Keisaku, Nakatani Kosuke, Takayama Kazuo, Tachibana Masashi, Mizuguchi Hiroyuki
Abstract excerpt
Clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9-mediated genome engineering technology is a powerful tool for generation of cells and animals with engineered mutations in their genomes. In order to introduce the CRISPR/Cas9 system into target cells, nonviral and viral vecto...
Topics
- A549 Cells
- Clustered Regularly Interspaced Short Palindromic Repeats
- DNA
- Efficiency
- Endonucleases
- Genetic Engineering
- Genetic Vectors
- Genome
- Genotype
- Humans
- Interferon Type I