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Article

Massive parallel insertion site sequencing of an arrayed Sinorhizobium meliloti signature-tagged mini-Tn 5 transposon mutant library.

Journal of biotechnology - 10 Sept 2017

Serrania Javier, Johner Tobias, Rupp Oliver, Goesmann Alexander, Becker Anke

Abstract excerpt

Transposon mutagenesis in conjunction with identification of genomic transposon insertion sites is a powerful tool for gene function studies. We have implemented a protocol for parallel determination of transposon insertion sites by Illumina sequencing involving a hierarchical barcoding method that allowed for tracking back insertion sites to individual clones of an arrayed signature-tagged transposon mutant...

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Source

Identifiers

PMID: 28235609
DOI: 10.1016/j.jbiotec.2017.02.019

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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