Article
Detecting the polymorphism of TERF1 gene by an improved PCR-RFLP method.
Journal of clinical laboratory analysis - 1 Jan 2018
Wang Pengpeng, Yang Yongli, Wang Sihua, Duan Xiaoran, Wang Tuanwei, Feng Xiaolei, Yan Zhen, Wu Yongjun, Yu Songcheng, Wang Wei
Abstract excerpt
BACKGROUND: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is a common and mature method of detecting the single nucleotide polymorphism (SNP). But, for the polymorphism site rs3863242 of telomeric repeat binding factor 1(TERF1) gene, there is no appropriate restriction enzyme to recognize it, which limits the research between the variants of rs3863242 and human diseases. METHODS:...
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