Article
Genome editing reveals dmrt1 as an essential male sex-determining gene in Chinese tongue sole (Cynoglossus semilaevis).
Scientific reports - 16 Feb 2017
Cui Zhongkai, Liu Yun, Wang Wenwen, Wang Qian, Zhang Ning, Lin Fan, Wang Na, Shao Changwei, Dong Zhongdian, Li Yangzhen, Yang Yingming, Hu Mengzhu, Li Hailong, Gao Fengtao, Wei Zhanfei, Meng Liang, Liu Yang, Wei Min, Zhu Ying, Guo Hua, Cheng Christopher H K, Schartl Manfred, Chen Songlin
Abstract excerpt
Chinese tongue sole is a marine fish with ZW sex determination. Genome sequencing suggested that the Z-linked dmrt1 is a putative male determination gene, but direct genetic evidence is still lacking. Here we show that TALEN of dmrt1 efficiently induced mutations of this gene. The ZZ dmrt1 mutant fish developed ovary-like testis, and the spermatogenesis was disrupted. The female-related genes foxl2 and cyp19a1a...
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