Article
Recent sequence variation in probe binding site affected detection of respiratory syncytial virus group B by real-time RT-PCR.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 1 Mar 2017
Kamau Everlyn, Agoti Charles N, Lewa Clement S, Oketch John, Owor Betty E, Otieno Grieven P, Bett Anne, Cane Patricia A, Nokes D James
Abstract excerpt
BACKGROUND: Direct immuno-fluorescence test (IFAT) and multiplex real-time RT-PCR have been central to RSV diagnosis in Kilifi, Kenya. Recently, these two methods showed discrepancies with an increasing number of PCR undetectable RSV-B viruses. OBJECTIVES: Establish if mismatches in the primer and probe binding sites could have reduced real-time RT-PCR sensitivity. STUDY DESIGN: Nucleoprotein (N) and glycoprotein...
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