Effect of Probe-Site Mismatches on Detection of Virulent Newcastle Disease Viruses Using a Fusion-Gene Real-Time Reverse Transcription Polymerase Chain Reaction Test

Abstract excerpt

Virulent forms of Newcastle disease virus (NDV) are a major concern for poultry producers around the world and the rapid diagnosis of an outbreak is crucial to any control program. A validated real-time reverse transcription-PCR test (fusion test) directed at the fusion-cleavage site of NDV was developed to differentiate virulent Newcastle disease virus strains from those of low virulence, however one virulent...

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