Article
A novel TaqMAMA assay for allelic discrimination of immunoproteasome subunit PSMB8 in pediatric patients.
Minerva pediatrics - 1 Jun 2022
Bergallo Massimiliano, Montanari Paola, Loiacono Elisa, Galliano Ilaria
Abstract excerpt
BACKGROUND: A more recent improvement in MAMA was made when the allele selectivity of MAMA primers was combined with the 5' fluorogenic exonuclease (TaqMan) assay. This strategy referred to as TaqMAMA. METHODS: A SNP rs9357155 at the PSMB8 locus (GenBank access number NM_148919.3.) on chromosome 6 showing a C/T transition in position 32842071, has been chosen as model in this study. RESULTS: Tested assays have...
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