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Article

Combining ancestral sequence reconstruction with protein design to identify an interface hotspot in a key metabolic enzyme complex.

Proteins - 1 Feb 2017

Holinski Alexandra, Heyn Kristina, Merkl Rainer, Sterner Reinhard

Abstract excerpt

It is important to identify hotspot residues that determine protein-protein interactions in interfaces of macromolecular complexes. We have applied a combination of ancestral sequence reconstruction and protein design to identify hotspots within imidazole glycerol phosphate synthase (ImGPS). ImGPS is a key metabolic enzyme complex, which links histidine and de novo purine biosynthesis and consists of the cyclase...

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PMID: 27936490
DOI: 10.1002/prot.25225

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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