Article
The properties and potential metabolic role of glucokinase in halotolerant obligate methanotroph Methylomicrobium alcaliphilum 20Z.
Antonie van Leeuwenhoek - 1 Mar 2017
Mustakhimov Ildar I, Rozova Olga N, Solntseva Natalia P, Khmelenina Valentina N, Reshetnikov Alexander S, Trotsenko Yuri A
Abstract excerpt
Aerobic bacteria utilizing methane as the carbon and energy source do not use sugars as growth substrates but possess the gene coding for glucokinase (Glk), an enzyme converting glucose into glucose 6-phosphate. Here we demonstrate the functionality and properties of Glk from an obligate methanotroph Methylomicrobium alcaliphilum 20Z. The recombinant Glk obtained by heterologous expression in Escherichia coli was...
Topics
- Bacterial Proteins
- Carbohydrate Metabolism
- Cloning, Molecular
- Enzyme Activation
- Escherichia coli
- Glucokinase
- Glucosyltransferases
- Glycogen
- Metabolic Networks and Pathways
- Methylococcaceae
- Mutation
- Phosphorylation
- Phylogeny