Article
Combination of two epitope identification techniques enables the rational design of soy allergen Gly m 4 mutants.
Biotechnology journal - 1 Feb 2017
Havenith Heide, Kern Karolin, Rautenberger Paul, Spiegel Holger, Szardenings Michael, Ueberham Elke, Lehmann Jörg, Buntru Matthias, Vogel Simon, Treudler Regina, Fischer Rainer, Schillberg Stefan
Abstract excerpt
Detailed IgE-binding epitope analysis is a key requirement for the understanding and development of diagnostic and therapeutic agents to address food allergies. An IgE-specific linear peptide microarray with random phage peptide display for the high-resolution mapping of IgE-binding epitopes of the major soybean allergen Gly m 4, which is a homologue to the birch pollen allergen Bet v 1 is combined. Three...
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