Article
Multiplex Single Nucleotide Polymorphism Analyses.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Hawkins Steve F C, Guest Paul C
Abstract excerpt
Quantitative polymerase chain reaction (qPCR) is a routinely used method for detection and quantitation of gene expression in real time. This is achieved through the incorporation and measurement of fluorescent reporter probes in the amplified cDNA strands, since the fluorescent signals increase as the reaction progresses. The availability of multiple probes that fluoresce at different wavelengths allows for...
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