Targeted Mutagenesis of <i>Burkholderia thailandensis</i> and <i>Burkholderia pseudomallei</i> through Natural Transformation of PCR Fragments

Abstract excerpt

Burkholderia pseudomallei is the causative agent of melioidosis, an overwhelming, rapidly fatal septic infection, and B. thailandensis is a closely related, less virulent species. Both organisms are naturally competent for DNA transformation, and this report describes a procedure exploiting this property for the rapid generation of marked deletion mutations by using PCR products. The method was employed to create...

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