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Article

Quantification of the Latent HIV-1 Reservoir Using Ultra Deep Sequencing and Primer ID in a Viral Outgrowth Assay.

Journal of acquired immune deficiency syndromes (1999) - 1 Feb 2017

Lee Sook-Kyung, Zhou Shuntai, Baldoni Pedro L, Spielvogel Ean, Archin Nancie M, Hudgens Michael G, Margolis David M, Swanstrom Ronald

Abstract excerpt

BACKGROUND: In this study, we measured the latent HIV-1 reservoir harboring replication-competent HIV-1 in resting CD4 T cells in participants on highly active antiretroviral therapy, quantitating the frequency of latent infection through the use of a Primer ID-based Ultra Deep Sequencing Assay (UDSA), in comparison to the readout of the quantitative viral outgrowth assay (QVOA). METHODS: Viral RNA derived from...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 27683060
DOI: 10.1097/QAI.0000000000001187
PMCID: PMC5233602

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