Article
Differences in vector-genome processing and illegitimate integration of non-integrating lentiviral vectors.
Gene therapy - 1 Jan 2017
Shaw A M, Joseph G L, Jasti A C, Sastry-Dent L, Witting S, Cornetta K
Abstract excerpt
A variety of mutations in lentiviral vector expression systems have been shown to generate a non-integrating phenotype. We studied a novel 12 base-pair U3-long terminal repeats (LTR) integrase (IN) attachment site deletion (U3-LTR att site) mutant and found similar physical titers to the previously reported IN catalytic core mutant IN/D116N. Both mutations led to a greater than two log reduction in vector...
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