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Infectious cDNA Clone of the Epidemic West Nile Virus from New York City

15 Jun 2002

Abstract excerpt

We report the first full-length infectious clone of the current epidemic, lineage I, strain of West Nile virus (WNV). The full-length cDNA was constructed from reverse transcription-PCR products of viral RNA from an isolate collected during the year 2000 outbreak in New York City. It was cloned into plasmid pBR322 under the control of a T7 promoter and stably amplified in Escherichia coli HB101. RNA transcribed...

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PMID: 12021317
DOI: 10.1128/jvi.76.12.5847-5856.2002

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