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Complete Human and Rat Ex Vivo Spermatogenesis from Fresh or Frozen Testicular Tissue

31 Aug 2016

Abstract excerpt

Until now, complete ex vivo spermatogenesis has been reported only in the mouse. In this species, the duration of spermatogenesis is 35 days, whereas it is 54 days in the rat and 74 days in humans. We performed long-term (until 60 days) cultures of fresh or frozen rat or human seminiferous tubule segments in a bioreactor made of a hollow cylinder of chitosan hydrogel. Testicular tissues were obtained from 8- or...

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PMID: 27580986
DOI: 10.1095/biolreprod.116.142802

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