Article
Screening for Escherichia coli K-12 genes conferring glyoxal resistance or sensitivity by transposon insertions.
FEMS microbiology letters - 1 Sept 2016
Lee Changhan, Kim Jihong, Kwon Minsuk, Lee Kihyun, Min Haeyoung, Kim Seong Hun, Kim Dongkyu, Lee Nayoung, Kim Jiyeun, Kim Doyun, Ko Changmin, Park Chankyu
Abstract excerpt
Glyoxal (GO) belongs to the reactive electrophilic species generated in vivo in all organisms. In order to identify targets of GO and their response mechanisms, we attempted to screen for GO-sensitive mutants by random insertions of TnphoA-132. The genes responsible for GO susceptibility were functionally classified as the following: (i) tRNA modification; trmE, gidA and truA, (ii) DNA repair; recA and recC,...
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