Article
Engineering a Prototypic P-type ATPase Listeria monocytogenes Ca(2+)-ATPase 1 for Single-Molecule FRET Studies.
Bioconjugate chemistry - 21 Sept 2016
Dyla Mateusz, Andersen Jacob Lauwring, Kjaergaard Magnus, Birkedal Victoria, Terry Daniel S, Altman Roger B, Blanchard Scott C, Nissen Poul, Knudsen Charlotte R
Abstract excerpt
Approximately 30% of the ATP generated in the living cell is utilized by P-type ATPase primary active transporters to generate and maintain electrochemical gradients across biological membranes. P-type ATPases undergo large conformational changes during their functional cycle to couple ATP hydrolysis in the cytoplasmic domains to ion transport across the membrane. The Ca(2+)-ATPase from Listeria monocytogenes,...
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