Article
Investigation of the functional role of human Interleukin-8 gene haplotypes by CRISPR/Cas9 mediated genome editing.
Scientific reports - 8 Aug 2016
Benakanakere Manjunatha R, Finoti Livia S, Tanaka Urara, Grant Gregory R, Scarel-Caminaga Raquel M, Kinane Denis F
Abstract excerpt
Interleukin-8 (IL-8) gene polymorphisms have been considered as susceptibility factors in periodontal disease. However, the functional roles of IL-8 gene haplotypes have not been investigated. Here, we demonstrate for the first time the use of the CRISPR/Cas9 system to engineer the IL-8 gene, and tested the functionality of different haplotypes. Two sgRNAs vectors targeting the IL-8 gene and the naked homologous...
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