Article
From clinical sample to complete genome: Comparing methods for the extraction of HIV-1 RNA for high-throughput deep sequencing.
Virus research - 15 Jul 2017
Cornelissen Marion, Gall Astrid, Vink Monique, Zorgdrager Fokla, Binter Špela, Edwards Stephanie, Jurriaans Suzanne, Bakker Margreet, Ong Swee Hoe, Gras Luuk, van Sighem Ard, Bezemer Daniela, de Wolf Frank, Reiss Peter, Kellam Paul, Berkhout Ben, Fraser Christophe, van der Kuyl Antoinette C
Abstract excerpt
The BEEHIVE (Bridging the Evolution and Epidemiology of HIV in Europe) project aims to analyse nearly-complete viral genomes from >3000 HIV-1 infected Europeans using high-throughput deep sequencing techniques to investigate the virus genetic contribution to virulence. Following the development of a computational pipeline, including a new de novo assembler for RNA virus genomes, to generate larger contiguous...
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