Article
Establishment of a nested-ASP-PCR method to determine the clarithromycin resistance of Helicobacter pylori.
World journal of gastroenterology - 7 Jul 2016
Luo Xiao-Feng, Jiao Jian-Hua, Zhang Wen-Yue, Pu Han-Ming, Qu Bao-Jin, Yang Bing-Ya, Hou Min, Ji Min-Jun
Abstract excerpt
AIM: To investigate clarithromycin resistance positions 2142, 2143 and 2144 of the 23SrRNA gene in Helicobacter pylori (H. pylori) by nested-allele specific primer-polymerase chain reaction (nested-ASP-PCR). METHODS: The gastric tissue and saliva samples from 99 patients with positive results of the rapid urease test (RUT) were collected. The nested-ASP-PCR method was carried out with the external primers and...
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