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Selection of housekeeping genes for real-time PCR in atopic human bronchial epithelial cells

16 Apr 2008

Abstract excerpt

The stability of housekeeping genes (HKGs) is critical when performing real-time quantitative PCR. To date, the stability of common HKGs has not been systematically compared in human airway epithelial cells (AEC) in normal and atopic subjects. Expression levels of 12 HKGs were measured in AECs from a cohort of 30 healthy atopic nonasthmatic or atopic asthmatic children. Gene expression stability was determined...

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PMID: 18417509
DOI: 10.1183/09031936.00129107

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