Preparation of Extracellular Matrices Produced by Cultured and Primary Fibroblasts.

doi:10.1002/cpcb.2

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Article

Preparation of Extracellular Matrices Produced by Cultured and Primary Fibroblasts.

Current protocols in cell biology - 1 Jun 2016

Franco-Barraza Janusz, Beacham Dorothy A, Amatangelo Michael D, Cukierman Edna

Abstract excerpt

Fibroblasts secrete and organize extracellular matrix (ECM), which provides structural support for their adhesion, migration, and tissue organization, besides regulating cellular functions such as growth and survival. Cell-to-matrix interactions are vital for vertebrate development. Disorders in these processes have been associated with fibrosis, developmental malformations, cancer, and other diseases. This unit...

Topics

Actins
Animals
Blotting, Western
Cattle
Cell Culture Techniques
Cell Separation
Cell Shape
Cells, Cultured
Extracellular Matrix
Fibroblasts
Humans
Mice
NIH 3T3 Cells
Phenotype
Serum Albumin, Bovine
Solubility
Staining and Labeling

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Identifiers

PMID: 27245425
DOI: 10.1002/cpcb.2
PMCID: PMC5058441

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