Article
Culture and phenotyping of chondrocytes in primary culture.
Methods in molecular medicine - 1 Jan 2004
Thirion Sylvie, Berenbaum Francis
Abstract excerpt
The culture of chondrocytes is one of the most powerful tool for exploring the intracellular and molecular features of chondrocyte differentiation and activation. However, chondrocytes tend to dedifferentiate to fibroblasts when they are subcultured, which is a major problem. This chapter describes several protocols for culturing chondrocytes of different anatomical origins (articular and costal chondrocytes)...
Topics
- Animals
- Cartilage, Articular
- Cattle
- Cell Culture Techniques
- Chondrocytes
- Humans
- Mice
- Phenotype
- Rabbits
- Rats
- Ribs
