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Fluorescence activated cell sorting followed by small RNA sequencing reveals stable microRNA expression during cell cycle progression

27 May 2016

Abstract excerpt

BACKGROUND: Previously, drug-based synchronization procedures were used for characterizing the cell cycle dependent transcriptional program. However, these synchronization methods result in growth imbalance and alteration of the cell cycle machinery. DNA content-based fluorescence activated cell sorting (FACS) is able to sort the different cell cycle phases without perturbing the cell cycle. MiRNAs are key...

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PMID: 27234232
DOI: 10.1186/s12864-016-2747-6

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