Article
Stereospecific capillary electrophoresis assays using pentapeptide substrates for the study of Aspergillus nidulans methionine sulfoxide reductase A and mutant enzymes.
Electrophoresis - 1 Jul 2016
Zhu Qingfu, El-Mergawy Rabab G, Zhou Yuzhen, Chen Chunyang, Heinemann Stefan H, Schönherr Roland, Robaa Dina, Sippl Wolfgang, Scriba Gerhard K E
Abstract excerpt
Stereospecific capillary electrophoresis-based methods for the analysis of methionine sulfoxide [Met(O)]-containing pentapeptides were developed in order to investigate the reduction of Met(O)-containing peptide substrates by recombinant Aspergillus nidulans methionine sulfoxide reductase A (MsrA) as well as enzymes carrying mutations in position Glu99 and Asp134. The separation of the diastereomers of the...
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