Article
[Application of Ion Torrent PGM™ System in Detection of Fetal DNA in Maternal Plasma].
Fa yi xue za zhi - 1 Dec 2015
Liu Ya-nan, Zhao Xue-ying, Ping Yuan, Xu Qing-wen, Huang Jiang-ping, Zou Kai-nan, Zhou Huai-gu
Abstract excerpt
OBJECTIVE: To explore the feasibility of detecting of Y-STR of fetal DNA in maternal plasma using Ion Torrent PGM™ System. METHODS: A total of 16 fetal DNA samples from maternal plasmas (8 cases from 38 weeks gestational age and 8 ones from 12 weeks) were prepared and a multiplex assay with 7 STR loci (DYS390, DYS391, DYS393, DYS438, DYS437, DYS456, DYS635) was designed for multiplex-PCR amplification. Using Ion...
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