Article
A somatic reference standard for cancer genome sequencing.
Scientific reports - 20 Apr 2016
Craig David W, Nasser Sara, Corbett Richard, Chan Simon K, Murray Lisa, Legendre Christophe, Tembe Waibhav, Adkins Jonathan, Kim Nancy, Wong Shukmei, Baker Angela, Enriquez Daniel, Pond Stephanie, Pleasance Erin, Mungall Andrew J, Moore Richard A, McDaniel Timothy, Ma Yussanne, Jones Steven J M, Marra Marco A, Carpten John D, Liang Winnie S
Abstract excerpt
Large-scale multiplexed identification of somatic alterations in cancer has become feasible with next generation sequencing (NGS). However, calibration of NGS somatic analysis tools has been hampered by a lack of tumor/normal reference standards. We thus performed paired PCR-free whole genome sequencing of a matched metastatic melanoma cell line (COLO829) and normal across three lineages and across separate...
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