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Three-dimensional direct measurement of cardiomyocyte volume, nuclearity, and ploidy in thick histological sections

6 Apr 2016

Abstract excerpt

Quantitative assessment of myocardial development and disease requires accurate measurement of cardiomyocyte volume, nuclearity (nuclei per cell), and ploidy (genome copies per cell). Current methods require enzymatically isolating cells, which excludes the use of archived tissue, or serial sectioning. We describe a method of analysis that permits the direct simultaneous measurement of cardiomyocyte volume,...

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PMID: 27048757
DOI: 10.1038/srep23756

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